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In vitro reactivity (“organ chamber”) of guinea pig tracheal rings—methodology considerations

   
@article{ATM10406,
	author = {Agnes Afrodite Sumarelli Albuquerque and Marco Túlio Menezes Carvalho and Patricia Martinez Evora and Tales Rubens de Nadai and Andrea Carla Celotto and Paulo Roberto Barbosa Evora},
	title = {In vitro reactivity (“organ chamber”) of guinea pig tracheal rings—methodology considerations},
	journal = {Annals of Translational Medicine},
	volume = {4},
	number = {11},
	year = {2016},
	keywords = {},
	abstract = {The present text was motivated by the difficulties faced by our postgraduate students when using airways studies protocols and will take into consideration the three mechanisms of relaxation: (I) guanosine 3,5-cyclic monophosphate (cGMP)/NO-dependent; (II) adenosine 3,5-cyclic monophosphate (cAMP)/PGI2-dependent, and (III) hyperpolarization-dependent. Tracheal rings are studied in an organ bath containing a gassed physiological salt solution, usually at a temperature of 37 ℃. An agent or procedure that causes contraction [acetylcholine (Ach) or metacholine] of the smooth muscle is needed before study airway dilator drugs. The presented airways studies protocols are useful to study the bronchial epithelial-dependent reactivity.},
	issn = {2305-5847},	url = {https://atm.amegroups.org/article/view/10406}
}