@article{ATM10967,
author = {Yi-Ting Yen and Jun-Neng Roan and Shih-Yuan Fang and Shi-Wei Chang and Yau-Lin Tseng and Chen-Fuh Lam},
title = {Autologous endothelial progenitor cells improve allograft survival in porcine lung transplantation with prolonged ischemia},
journal = {Annals of Translational Medicine},
volume = {4},
number = {15},
year = {2016},
keywords = {},
abstract = {Background: As endothelial progenitor cells (EPCs) attenuated acute lung injury (ALI) in rabbit model, we hypothesized that autologous EPCs preserved lung graft function during the acute reperfusion period of lung transplantation and tested the therapeutic potential of EPCs in a porcine model of lung transplantation with prolonged graft ischemia.
Methods: Day-7 EPCs isolated from the recipient subjects or plain culture media were administered into the left pulmonary artery immediately before restoration of pulmonary blood flow in a porcine lung allotransplantation model, with the transplantation surgeons blinded to the content of injection. Hemodynamics and arterial blood gas were recorded, and the right pulmonary artery was occluded 30 min after reperfusion to evaluate the lung graft function. The lung grafts were sectioned for histological examination at the end of experiments. The total ischemic time for lung graft was approximately 14 h.
Results: All animals receiving plain medium died within 40 min after reperfusion, but 3 out of 5 (60%) piglets receiving EPCs survived up to 4 h after diversion of the entire cardiac output into the lung graft (P},
issn = {2305-5847}, url = {https://atm.amegroups.org/article/view/10967}
}