@article{ATM4297,
author = {Jun Liang and Zhaoqiang Zhang and Lizhong Liang and Yun Shen and Kexiong Ouyang},
title = {HIF-1α regulated tongue squamous cell carcinoma cell growth via regulating VEGF expression in a xenograft model},
journal = {Annals of Translational Medicine},
volume = {2},
number = {9},
year = {2014},
keywords = {},
abstract = {Objective: We aimed to study the mechanism of hypoxia-inducible factor 1 alpha (HIF-1α) regulating the cell proliferation of tongue squamous cell carcinoma (TSCC) via vascular endothelial growth factor (VEGF).
Methods: We used RNA interference (RNAi) technique, transfected chemically synthesized siRNA against HIF-1α into CAL-27 cells, and detected the expression of HIF-1α and VEGF by real time-PCR and Western blotting in order to find out if HIF-1α regulated the expression of VEGF. A xenograft experiment was carried out to observe the role of HIF-1α on the tumor growth of tongue squamous cell carcinoma.
Results: HIF-1α and VEGF mRNA expression was significantly downregulated 36 and 48 h after transfection (P<0.05); the protein expression of HIF-1α and VEGF was also significantly suppressed by siRNA against HIF-1α. Furthermore, intratumoraly injection of HIF-1α targeting siRNA suppressed tumor growth in nude mice.
Conclusions: HIF-1α regulated VEGF expression, and they may contribute to TSCC cell tumor growth.},
issn = {2305-5847}, url = {https://atm.amegroups.org/article/view/4297}
}