Erratum to LXA4 protects against blue-light induced retinal degeneration in human A2E-laden RPE cells and Balb-c mice

Erratum to: Ann Transl Med 2021;9:1249.

This article (1) titled “LXA4 protects against blue-light induced retinal degeneration in human A2E-laden RPE cells and Balb-c mice” (doi: 10.21037/atm-21-3390), unfortunately contains an error in Figure 7F. In Figure 7F, the image which selected to represent the dihydroethidium (DHE) staining in the retinal pigment epithelium (RPE) cells of the A2E+B15+LXA4+siNRF experimental group was mistakenly labeled as the fluorescence staining images of the A2E+B1 experimental group in Figure 3A. After thoroughly re-examining the original datasets of the DHE staining results for Figure 7F, the data was re-quantified. Thus “N=4” in the legend of Figure 7G should be changed to “N=6”. The corrections are presented below.

Figure 7F of the original article:

Corrected Figure 7F:

The correct version of Figure 7, containing the correct data for the quantification of DHE-positive cells post BL illumination in Figure 7G, is shown below.

Figure 7 Blocking the NRF2-HO1 pathway reverses the protective effects of LXA4 on BL-induced ROS elimination. (A,B) The protein levels of cytoplasmic NRF2, HO1, and NQO1. Representative blots are shown, with quantification; N=4. (C,D) The levels of nuclear NRF2 were determined; N=4. (E) The mRNA expression of HO1; N=4. (F) Representative images of DHE staining; scale bar, 50 µm. (G) Quantification of DHE-positive cells post BL illumination; N=6. Con, vehicle control; A2E + B15, RPE cells treated with A2E and exposed to BL for 15 h; A2E + B15 + LXA4, RPE cells treated with A2E and exposed to LXA4 pre-treatment for 30 min and BL for 15 h; A2E + B15 + LXA4 + siNRF, RPE cells treated with A2E after transfected with NRF2 siRNA exposed to LXA4 pre-treatment for 30 min and BL for 15 h. The results are presented as the mean ± SEM, *P<0.05 and **P<0.01. RPE, retinal pigment epithelial (/epithelium) layer.

The authors confirmed this error did not significantly affect either the results or the conclusions of the paper.

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References

1. Xie T, Cai J, Yao Y, et al. LXA4 protects against blue-light induced retinal degeneration in human A2E-laden RPE cells and Balb-c mice. Ann Transl Med 2021;9:1249. [Crossref] [PubMed]