Erratum to Oxymatrine attenuates arsenic-induced endoplasmic reticulum stress and calcium dyshomeostasis in hepatic stellate cells

Erratum to: Ann Transl Med 2020;8:1171.

The article (1) titled “Oxymatrine attenuates arsenic-induced endoplasmic reticulum stress and calcium dyshomeostasis in hepatic stellate cells” (doi: 10.21037/atm-20-5881) unfortunately contains errors in Figures 1-4 while the figure legends remain intact. The molecular weight of GRP78 and α-SMA were wrongly labelled. The molecular weights of GRP78 should be 78 kDa, not 37 kDa on Figure 2C and Figure 3A. The α-SMA should be 42 kDa, not 72 kDa on Figure 1F, Figure 3C and Figure 4D.

Figures 1-4 of the original article:

The correct version of Figures 1-4, containing the correct molecular weights of GRP78 and α-SMA in Figures 1-4, is shown below:

Figure 1 Oxymatrine suppresses NaAsO₂-induced LX2 activation and ECM secretion. (A,B) LX2 cell proliferation determined with CCK-8 assay after exposure to NaAsO₂ and OM in a dose- and time-dependent manner. (C) Flow cytometry analysis of LX2 cells after exposure to NaAsO₂ and OM. (D,E) HA, COL-IV, and TGF-β1 expression was determined using ELISA. (F) Western blotting and statistical analysis of α-SMA protein level. (G,H) SOD and MDA expression after NaAsO₂ and OM exposure. Data are presented as the mean ± SD, n=6, *, P<0.05 vs. control; ^#, P<0.05 vs. NaAsO₂ group. ECM, extracellular cell matrix; CCK-8, Cell Counting Kit-8; OM, oxymatrine; α-SMA, α-smooth muscle actin; HA, hyaluronic acid; COL-IV, collagen type IV; SOD, superoxide dismutase; MDA, malondialdehyde.

Figure 2 Oxymatrine suppresses NaAsO₂-induced endoplasmic reticulum stress. (A,B) The relative mRNA of CHOP, GRP78, SERCA2, RyR1 expression after exposure to NaAsO₂ and OM in LX2 cells. (C) Western blotting and statistical analysis of Calpain2, CHOP, GRP78, and SERCA2. (D) Calcium concentration in LX2 cells after exposure to NaAsO₂ and OM. Data are presented as the mean ± SD, n=6. *, P<0.05 vs. control; ^#, P<0.05 vs. NaAsO₂ group. OM, oxymatrine; CHOP, DNA damage-inducible transcript 3 protein; RyR1, ryanodine receptor.

Figure 3 Overexpression of GRP78 blocks NaAsO₂ effects in LX2 cells. (A) Western blotting and statistical analysis of GRP78 to confirm the effect of GRP78-shRNA and GRP78-Overexpression plasmid transfection. (B) Flow cytometry analysis of LX2 cells after GRP78 plasmid transfection and NaAsO₂ exposure. (C) Western blotting and statistical analysis of cleaved caspase 12, CHOP, and α-SMA. (D,E) Expression of TGF-β1, HA, and COL-IV after NaAsO₂ and GRP78 plasmid transfection and NaAsO₂ exposure in LX2 cells. Data are presented as the mean ± SD, n=6. *, P<0.05 vs. shRNAcon; ^&, P<0.05 vs. vector; ^#, P<0.05 vs. NaAsO₂ group. C-Cas 12, Cleaved-Caspase 12; CHOP, DNA damage-inducible transcript 3 protein; α-SMA, α-smooth muscle actin; HA, hyaluronic acid; COL-IV, collagen type IV; shRNA, short hairpin RNA.

Figure 4 SERCA2 knockdown blocks NaAsO₂-induced effects in LX2 cells. (A) Western blotting and statistical analysis of SERCA2 to confirm the effect of SERCA2-shRNA and SERCA2-Overexpression plasmid transfection. (B) The relative mRNA of CHOP, GRP78, SERCA2 after shRNA, and Overexpression plasmid transfection. (C,D) Western blotting and statistical analysis of C-caspase12 and α-SMA. (E,F) Expression of TGF-β1, HA, and COL-IV after NaAsO₂ and SERCA2 plasmid transfection in LX2 cells. (G) Intracellular calcium concentration after SERCA2 knockdown and overexpression, NaAsO₂, and OM treatment. Data are presented as the mean ± SD, n=6. *, P<0.05 vs. shRNAcon; ^&, P<0.05 vs. vector; ^#, P<0.05 vs. NaAsO₂ group. C-Cas 12, Cleaved-Caspase 12; CHOP, DNA damage-inducible transcript 3 protein; shRNA, short hairpin RNA; α-SMA, α-smooth muscle actin; HA, hyaluronic acid; COL-IV, collagen type IV; OM, oxymatrine.

The authors confirmed these errors did not significantly affect either the results or the conclusions of the paper.

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References

1. Wang H, Han B, Wang N, et al. Oxymatrine attenuates arsenic-induced endoplasmic reticulum stress and calcium dyshomeostasis in hepatic stellate cells. Ann Transl Med 2020;8:1171. [Crossref] [PubMed]