Review Article


In vitro reactivity (“organ chamber”) of guinea pig tracheal rings—methodology considerations

Agnes Afrodite Sumarelli Albuquerque, Marco Túlio Menezes Carvalho, Patricia Martinez Evora, Tales Rubens de Nadai, Andrea Carla Celotto, Paulo Roberto Barbosa Evora

Abstract

The present text was motivated by the difficulties faced by our postgraduate students when using airways studies protocols and will take into consideration the three mechanisms of relaxation: (I) guanosine 3,5-cyclic monophosphate (cGMP)/NO-dependent; (II) adenosine 3,5-cyclic monophosphate (cAMP)/PGI2-dependent, and (III) hyperpolarization-dependent. Tracheal rings are studied in an organ bath containing a gassed physiological salt solution, usually at a temperature of 37 ℃. An agent or procedure that causes contraction [acetylcholine (Ach) or metacholine] of the smooth muscle is needed before study airway dilator drugs. The presented airways studies protocols are useful to study the bronchial epithelial-dependent reactivity.

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